ABOUT US

Veterinary Biological and Research Institute (VBRI) was established in 1944 as serum institute to cater the health needs of livestock and poultry population of AP state. Further it has been evolved in to one of the leading government organization in veterinary vaccine production and disease investigation.

Vaccine production facilities are located at two places

1. Hyderabad -   Producing 10 types of Bacterial and Viral vaccines for livestock & poultry

2. Samalkot    -   Producing 5 types of vaccines
(East Godavari district)

 The disease investigation facilities are located at various places 

 1. State referral laboratory at Hyderabad 
2. District laboratories located at district head quarters in each district 

Vaccine production facility

The VBRI is catering to the vaccine needs of live stock and poultry of Andhra Pradesh state. The institute is contemplating for modernisation of all vaccine production laboratories as per cGMP standards.  Modernisation of Rabies tissue culture Vaccine and Poultry vaccines production laboratories is completed and in other laboratories work is under progress.

Anti Rabies tissue culture vaccine facility

Aims and objectives       

  • To produce various biologicals for controlling the livestock and poultry diseases by conducting prophylactic vaccinations.
  • To provide prompt and accurate diagnostic services which enable field veterinarians to control the diseases effectively
  • To undertake epidemiological studies of animal diseases in the state with a view to make plans for eradication of the diseases.
  • To introduce new techniques for  developing  vaccines and diagnostic aids

Contact address for vaccine production facility

Joint Director (AH)
Veterinary Biological and Research Institute
Shanthinagar, Hyderabad -500028
Telephone No – 040- 23316366
Fax No- 040- 23307982
Email address- vbri_ahd@ yahoo.com

Contact address for Disease investigation facility

Joint Director (AH)
Disease investigation
Veterinary Biological and Research Institute
Shanthinagar, Hyderabad -500028
Telephone No – 040- 23376016
Fax No           - 040- 23376016

Contact address for vaccine production facility

Deputy Director (AH)
Veterinary Biological and Research Institute
Samalkot
Phone no.

Organogram of Veterinary Biological & Research Institute

Organization Chart 

Disease Investigation Wing

Disease Investigation Wing works as reference laboratory for Disease Diagnosis and Investigation of disease condition in livestock, canines, captive wild animals, poultry etc. through network of Animal Disease Diagnostic Laboratories located in each district.

The Disease Investigation Wing consists of following sections

  • Cattle Disease Investigation
  • Sheep and Goat Disease Investigation
  • Poultry Disease Investigation
  • Foot & Mouth Disease Laboratory
  • Brucellosis Diagnosis Laboratory
  • Japanese Encephalitis Laboratory
  • Tuberculosis & Johnes Disease Laboratory
  • Microbiology Laboratory
  • Pathology Laboratory
  • Helminthology Laboratory
  • Toxicology & Feed Analysis Laboratory

Salient features and achievements of Disease Investigation wing of VBRI

    • This Institute was recognized by Govt. of India as collaborating center of ADMAS, Bangalore.
    • FMD lab was also recognized for FMD sero typing center of Govt. of India
    • In JE Lab, human sera samples are analyzed along with sera samples of pigs and other animals.
    • Brucellosis lab has taken up the study on prevalence of brucellosis in veterinarians & para veterinarians and domestic animals.
    • Screening of Tuberculosis & Johnes Diseases of animals in the government livestock farms and other livestock farms is done regularly.
    • Detection of Aflatoxin, HCN, Nitrates, Nitrites, Lead is done in Toxicology lab.
    • Food Microbiology Lab is also established for assessment of Microbial quality of meat, eggs samples etc.
    • Fluorescent Antibody Assay for Rabies Disease Diagnosis is also established.
    • Quality Assessment lab is also being established, which will undertake the detection of antibiotics, pesticide and hormone residues.
    • Training is being imparted to all the field staff on preparedness for Avian Influenza, Disease diagnosis and Disease control.

DISEASE DIAGNOSIS / INVESTIGATION

Laboratory Diagnosis: The laboratory awareness of a field Veterinarian as well as prompt responsiveness by laboratory worker to a field difficulty are the best symbiotic actions expected.  The results of laboratory examination has bearing on following actions of collection and forwarding the material.

  • Correct sampling
  • Appropriate preservation
  • Precise labeling and identification
  • Covering letter
  • Careful packing

Sampling: The starting point for the laboratory investigation of an animal disease is the taking of a sample.  Samples may be taken from animals, or their environment, for a variety of purposes. These may be for helping to establish a disease diagnosis, for health surveillance, for health certification or for monitoring the response to treatment or vaccination.  Samples must be taken with care, to avoid undue stress or damage to the animal or danger to the operator. It is usually important to adopt aseptic techniques, and care must be taken to avoid cross-contamination between samples.

Having obtained suitable material, it must be carefully packaged, labelled, and transmitted to the laboratory by the fastest practicable method, preferably with temperature control. Before taking samples, careful consideration must be given to the purpose for which they are required.

Type of sample: Considerable skill and care are required to decide on the correct samples to be sent to the laboratory. It depends of the tentative clinical diagnosis and material is to be selected accordingly.  Not all materials are required for all the diseases.
Samples may be classified as per examination point of view.

  • Bacteriological / Virological /  Mycological
  • Immunological / Serological
  • Parasitological
  • Pathological / Histopathological
  • Toxicological

1. Blood: Blood samples may be taken for haematology or for culture and/or direct examination for bacteria, viruses, or protozoa, in which case it is usual to use anticoagulants.  They may also be taken for serology, in which case a clotted sample is required. In most large mammals, the jugular vein or a caudal vein is selected, but brachial veins and mammary veins are also used. In birds, a wing vein (brachial vein) is usually selected. Small quantities of blood are conveniently obtained by pricking with a triangular, solid-pointed needle. Ideally the skin at the site of venepuncture should first be shaved (plucked) and swabbed with 70% ethyl alcohol and allowed to dry. For samples with anticoagulant and/or antibiotics, thorough mixing is necessary as soon as the sample has been taken. It may also be necessary to make a smear of fresh blood on a microscope slide; both thick and thin smears may be prepared.

2. Faeces: Freshly voided faeces of suitable quantity (about 5-10 g) should be selected, and sent with or without a transport medium. Faeces for parasitology should fill the container or be topped up with sterile water to reduce air and prevent hatching of parasite eggs. An alternative and sometimes preferable method is to take swabs from the rectum (or cloaca), taking care to swab the mucosal surface. The swabs should be visibly coated with faecal material. Care must be exercised when taking swabs from small, delicate animals or birds to avoid injuring them. Swabs may be transported either dry or in transport medium. Faeces are best stored and transported at 4°C.

3. Skin scrapings: Specimens for the diagnosis of fungal skin infections should be taken from edges of affected skin areas or tissues of the animals aseptically.  Part of the tissue should be placed in 10% formaline solution for histological examination.  For mange mites and other infestations deep skin scrapings till the area is reddened is essential . Plucked hair or wool samples may be useful for certain dermatological infections. They can be packed in dry paper without preservative.

4. Vesicular fluid:  In viral diseases producing vesicular rashes or where lesions are exclusively in the skin, samples are taken from the lesions themselves. Scrapings of the lesion may be taken; 2 g of affected epithelial tissue is taken as aseptically as possible and placed in 5 ml phosphate buffered glycerin virus transport medium at pH 7.6.

5. Genital tract and semen: Samples may be taken by vaginal or preputial washing, or by the use of suitable swabs. Sometimes the cervix or urethra is also sampled by swabbing. Samples of semen are best obtained using an artificial vagina or by artificial stimulation.

6. Eye: A swab of the surface of the conjunctiva may be taken gently, holding the palpebra apart. The swab is then broken off into transport medium

7. Nasal discharge (saliva, tears): It may be helpful if they are first wetted with transport medium. They should be allowed to remain in contact with the secretions for up to 1 minute, then placed in transport medium and sent to the laboratory without delay at 4°C.

8. Milk: Samples of milk should be taken after cleansing and drying the tip of the teat, but antiseptics should be avoided at this time. The initial stream of milk should be discarded and a tube filled with the next stream (s).

9. Environment and feed: Environmental samples are commonly taken from litter or bedding and voided faeces or urine. Swabs may be taken from the surface of ventilation ducts (which are usually dusty), feed troughs and drains. This kind of sampling is particularly important in hatcheries, artificial insemination centres and slaughter houses in which specialised equipment is maintained

10. Tissues: may be collected for microbiological culture, parasitology, biochemistry, histopathology and/or immuno-histochemistry.  Freezing at -20°C may be detrimental to virus isolation. The tissues may be sent to the laboratory dry or in bacterial or virus transport medium, depending on the examinations required. For histopathology, blocks of tissue not more than 0.5 cm thick and 1-2 cm2 are cut and placed in neutral buffered       4-10% formalin, which should be at least ten times the volume of the tissue sample. Samples for histology should not be frozen. Once fixed, tissues can be removed from formalin and, as long as they are kept moist and protected (e.g. by wrapping in formalin-soaked paper towels, then sealed in screw-capped jars), they can be forwarded to the laboratory without formalin.

Sample size :  There are some general statistical rules that should be borne in mind, particularly when sampling herds or flocks for a health surveillance scheme. It is possible to calculate how many animals must be sampled from a herd/flock of a certain size, to achieve a 95% probability of detecting infection assumed to be present in a certain percentage of the animals. For example, if disease were present in 5% of a herd of 500 animals, it would be necessary to sample 56 animals to be 95% confident of finding one positive, assuming that both the sensitivity and specificity of the test were 100%.

Information to be sent with samples : It is essential that samples be clearly identified using appropriate methods. Marking instruments must be able to withstand the condition of use, i.e. being wet or frozen. Pencil has a tendency to rub off containers and labels attached to plastic will fall off when packs are stored at -70°C. Information and case history should always accompany the samples to the laboratory, and preferably should be placed in a plastic envelope. The information should include the following points:


1.   

Name and address of owner where disease occurred and date of despatch,

2.   

Disease(s) suspected,

3.   

Samples submitted, tests required (transport medium used) and date of sampling,

4.   

Different species on the farm and number, age and sex of each affected animal, identification numbers,

5.   

Length of time on the farm; if recent arrival, where from,

6.   

Date of first cases and of subsequent cases or losses, with any appropriate previous submission reference numbers,

7.   

Description of the spread of infection in the herd or flock,

8.   

Number of animals dead, the number showing clinical signs, and their age, sex and breed,

9.   

The clinical signs and their duration including the condition of mouth, eyes and feet, and milk or egg production data,

10.   

Type and standard of husbandry, including the type of feed available, possible contact with poison or poisonous plants,

11.   

A list and description of the samples examined post-mortem with date of examination and the observed findings,

12.   

Any medication already applied to the animals, and when given,

13.   

Any vaccination already given, and when given,

14.   

Name and address of the sender, with telephone and fax number, and date of submission.

Packing and labeling: Packing and labeling are very important before dispatching the materials for laboratory diagnosis. Suitable packing materials are to be selected. Also labeling should be done precisely on containers with legible ink that do not blot or labels that do not get pealed off in wet conditions.  The slides should be labeled with diamond glass marker pens preferably.
Transport of samples :  Samples must be carefully packed, to avoid any possibility of leakage or cross-contamination. They should be delivered within 48 hours and should usually be kept cool during transit. Some samples should not be frozen. Screw-capped bottles should be used and should be additionally sealed with adhesive tape or paraffin wax. Samples in individually identified containers should be placed in larger strong outer containers and packed with enough absorbent material to protect from damage.

General instructions for sending the samples to the diagnostic laboratory  :

  • From cases of an outbreak at least 4-5 specimens be collected. Fresh carcass be autopsied.
  • All specimens collected for Bacteriological/ Virus isolation must be collected aseptically and dispatched on ice with courier with instructions to change ice on the way.
  • Invariably collect the materials for isolation of etiological agents as well as in 10% formaline for histopathology for complete diagnosis.
  • For parasitic diseases collect blood smears and stools/ faeces from 10-15 animals. Put a drop of formaline in stools and fix smears with alcohol.  
  • Most of the known viruses affecting animals have a tendency for selective tissue localization. This factor demands the utmost care in selecting the specimens appropriate for the disease and as free as possible from bacterial contamination. Tissue specimens may be placed in sterile wide-mouthed cork stoppered bottles and shipped frozen in dry ice or in 5 to 10 volumes of sterile 50% buffered glycerine saline refrigerated in transit.

Common samples sent to laboratory examinations for different investigations   

1.  BLOOD SMEARS       :             Bacteriological, Protozological and pathological
( 2 thin and 2 thick smears)
2.  WHOLE BLOOD         :            Bacteriological or Virological
( Without preservative preferably on ice)
3.  CITRATED BLOOD    :            Pathological
( Refrigerated  4 – 10 C)
4.  SERUM                         :            Serum is useful component in the absence of clinical  
infection for Serodiagnosis
( Non hamolysed
5. URINE                           :            Bacteriological, Pathological
( Fresh uncontaminated urine, from mid stream )
6. FAECES                          :            Parastological and Bacteriological
7. SKIN SCRAPINGS         :           Mycological and parasitological
8.  TISSUE                           :           Histopathological
( Collect from fresh carcass , affected  with                                                                           adjoining healthy  tissue should be included)
9. IMPRESSION SMEARS :           Bacteriological , Negri bodies
(Should be collected from affected tissue. Cut and                                                               after blotting  on a clean blotting paper gently press                                                              on a clean  slide. Take 2 to 3 smears from one tissue                                                       and after dried, wrap in paper)
10. HEART BLOOD            :            Bacteriological
(When a fresh carcass is presented for autopsy, for   
bacterial isolation , it is the easiest way to collect
heart blood in sterile Pasteur-pipette and sent to
laboratory after proper sealing.)

Precautions for collecting materials for Microbiological examination :

  • Material for Bacteriological or Virological examination should be obtained as free from contamination as possible.
  • A bunsen burner / spirit lamp may be used for collection of specimen aseptically and transfer to sterile container.
  • Suitable instruments including scalpels, scissors and forceps in sufficient number must be assembled and sterilized.
  • A convenient and effective method of sterilization is to immerse the instruments in boiling water containing 2% sodium carbonate for 15 minutes.
  • A basin containing antiseptic solution should be available for rinsing the gloved hands at different steps during the autopsy.
  • Before primary incision is made, the skin of the surrounding area should be disinfected with a 2% solution of cresol.
  • Material from body cavities for bacteriological study is obtained in a manner which will not contaminate others. 
  • Generous blocks of liver, spleen, kidneys, lymph nodes, lungs, intestinal loop and brain should be removed with sterile instruments and placed in sterile bottles for transport.
  • Heart blood may be obtained with sterile syringe or pipette after searing the surface of heart with hot spatula and shipped in sterile vials. 
  • Liberal amounts of pus and exudate must be collected on sterile cotton swabs and shipped in sterile test tubes. 
  • Pleural, pericardial, joint and cerebrospinal fluid should be collected with sterile syringe and needle and shipped in sterile leak proof vials. 
  • Thin blood smears and pus smears on clean glass slides are often of assistance in making a Laboratory diagnosis. 
  • It is essential that material for bacteriological examination must be held under refrigeration from time of collection until received in laboratory.
  • This may be accomplished by the use of ordinary ice or solid co2 (Dry ice).

Precautions for collecting materials for Serological examination :  Non haemolysed blood serum is preferable for serological tests. Freezing of whole blood should be avoided since haemolysis may render the specimen unsuitable for use. To obtain clear serum, withdraw 10ml of blood in a dry sterile syringe from jugular vein of an animal or from right auricle or ventricle of one that recently died. After detaching the needle from the syringe, transfer the blood to a sterile test tube to clot. Refrigerate overnight, with draw serum (Centrifuge if required) and place in sterile vial. Add preservative as per recommendation (antibiotic or Merthiolate) and dispatch for examination. (Vacutainer is preferable for collection of blood for serological tests)

Precautions for collecting materials for virological examination: Most of the known viruses affecting animals have a tendency for selective tissue localization. This factor demands the utmost care in selecting the specimens appropriate for the disease and as free as possible from bacterial contamination. Tissue specimens may be placed in sterile wide-mouthed cork stoppered bottles and transported frozen in dry ice or odinary ice.  Heart blood, cerebrospinal fluid for identification of virus should be obtained under aseptic conditions and shipped in sterile vials refrigerated with dry ice.

Precautions for collecting materials for Histopathological studies :  For histopathology, blocks of tissue not more than 0.5 cm thick and 1-2 cm2 are cut and placed in 4-10% formalin, which should be at least ten times the volume of the tissue sample. Samples for histology should not be frozen.

Transport medium / 50 % Phosphate buffered glycerine (pH 7.4)
Sodium chloride                                 8.0 gms
Potassium chloride                              0.2 gms
Di-sodium hydrogen phosphate          1.15 gms
Potassium dihydrogen phosphate       0.20 gms
Distilled water                                    1000 ml          

Add equal volume of sterile neutral glycerine to sterile PBS to prepare 50% phosphate buffered glycerine. Add 0.1 ml of 15 phenol red solution to 100 ml of this solution to get a final concentration of 0.001%.  When sterile the solution will have reddish tinge and upon contamination turns yellow in colour.

10% Formalin saline solution

   

Sodium chloride

8.5 gms

Formalin (40%)

100 ml

Double distilled water        

900 ml

 Oxalate phenol glycerine (OCG) solution              


Potasium oxalate

500 ml

Glycerine

500 ml

Carbolic acid

5gms

Distilled water

500 ml

DIAGNOSTIC MATERIALS FOR BACTERIAL DISEASES OF LIVESTOCK

DISEASE                    MATERIALS TO BE COLLECTED            PRESERVATIVE

Actinobacillosis                  a.  Pus from affected organ                              On ice

Actinomycosis                    b. Pus smears
c. a small piece of affected tissue                     10% formalin

Anthrax                              Do not conduct postmortem
a. Collect blood smears from ear vein
( within 6 hours of death)
b. Ear piece
c. If accidentally the carcass is
opened, collect a small piece if
spleen and dispose the carcass
in deep-coated pit.

Black quarter`                    Do not send blood smears.
a. Exudate from crepitating swelling                    On ice
b. Muscle piece from affected area                     In salt
c. Exudate smears

Haemorrhagic                    a. exudate  smears from throat swelling
septicaemia                         b. Blood smears , 2 thick and 2 thin
c. Heat blood swab                                              On ice
d. Piece of spleen and lung from carcass             On ice

Mastitis                               About  5-8 ml of milk from affected quarter       On ice
Discard first few strips of milk

Infectious abortions           The material has to be collected from
Due to                                 Dam – Swab from uterine discharge
Brucellosis                                       Cervical swab
Vibriosis                                           Smears from vaginal discharge
Trichomoniasis                                Serum samples after 21 days
Leptospirosis                      Aborted foetus
Mycoses                                            - Stomach contents aseptically                  On ice
Chlamydiasis                                    if fresh foetal membranes                         On ice
Pleural fluid, kidney and liver                  On ice
Sire -  Serum samples
Preputial washings, Semen

Pleuropneumonia               a. Piece of lung,  Pleural fluid,                           On ice
(CBPP,  CCPP)                   b. Mediastinal lymph gland
c. Serum
d. Piece of lung                                                        10% formalin

Tuberculosis                         a. Suspected lymph node biopsy in Live animals     On ice
b. Portions of affected organs showing
Caseous / necrotic lesions, associated                   50%  GPB
lymph glands
c. Impression smears from lesions
d. Pieces of liver, spleen, kidney, lungs                    10% formalin

Johne’s disease                    a.  Rectal pinch smears, faecal sample
b.  Affected portions of intestines                                 10% formaline
in case  dead animal ( ileocaecal valve)
c. Mesentric lymph node smears

Leptospirosis                       a.  Serum samples
b. Freshly voided urine
c. Whole blood at height of temperature                       On ice
d. Kidney and liver                                              25%  GPB
e. Blood smears and urine smears

Lister                                    a. Brain                                                                           On ice
b. Cerebrospinal fluid                                                     On ice
c. serum

Enterotoxaemia                  a. Intestinal loop  12”                                               0.5 %  Chloroform

  • Urine sample

    
DIAGNOSTIC MATERIALS FOR VIRAL DISEASES OF LIVESTOCK

FMD                                a. Vesicular fluid                                                          On ice
Vesicular exanthema        b. Epithelial lining / buccal mucosa from lesions        50% GPB
Vesicular Stomatitis                      

Rinderpest / PPR              a.  Whole blood in heparin or citrate                                       On ice
b. Spleen and mesenteric lymph nodes                              On ice           
c. Swabs from nasal, buccal, conjunctival                         On ice           
discharges, rectal mucosa (PPR)
                                            d. Lung piece  (PPR)                                                          On ice

Blue tongue                       a. Heparinised blood  ( Viraemic stage)                                        
b. Whole blood                                             OCG  in equal parts
c. Serum samples without preservative
d. Spleen                                                              50 % GPB/ On ice

IBR / Virus pneumonia/    a. Nasal swab / Nasal discharge                             On ice
Influenza                           b. Lung piece                                                               On ice
c. Lung piece                                                       10% formalin
d. Serum  (IBR)

Bovine Malignant Catarrh   Brain, lymph node, liver, Kidney                                  10% formalin

Bovine Viral diarrhoea /      a. Whole blood at thermal phage                                  On ice
Mucosal disease                    b. Spleen and mesenteric ly. Node                                On ice 
c. Serum

Pox / Contagious ecthyma /     a. Skin lesions                                       Dry in vial/50% GPB
Contagious pustular dermatitis

Sheep Pox                              a. Scabs                                                        50 % GPB / 10% formalin           

Hog cholera                          a. Whole blood                                                              On ice
b. Spleen and mesenteric lymph node                           On ice            
c. Piece of small intestine                                             10% formaline
Equine infectious anaemia      Whole blood                                                                  On ice

African horse sickness             a. Heparinised blood                                        On ice
b. Spleen                                                             OCG / ice
c. Serum samples

Rabies/ Pseudorabies            a. Brain specifically labelled                              On ice
b. Piece of brain                                                10% formaline
c. Impression smears from Ammon’s horn of brain

Canine distemper                    a. Whole blood                                                  On ice           
b. Brain, spleen and lung                                 50% GPB      
c. Brain and lung piece                                             10% formaline

Infectious canine hepatitis     a. Liver, spleen                                                         On ice    
b. Mediastinal lymph node                                     On ice
c. Urine                                                                  On ice    
d. Liver                                                           10% formaline

Canine Parvovirus                  Mesenteric lymph node                                             On ice

DIAGNOSTIC MATERIALS  FOR DISEASES OF POULTRY

Salmonellosis ( BWD)            a. Liver and Spleen                                                     On ice
Fowl cholera                          b. Whole blood                                                 Without preservative
Colisepticaemia                     c. Fresh dead bird
d. Serum  ( BWD)

Spirochetosis                            a. Blood smear
b. Spleen                                                                    On ice
c. Ailing bird
d. Ticks from affected bird

Aspergillosis                          a. Lung, caseous masses from airsac         
b. Lung, trachea                                              10% formaline
c. Smears from abcesses

Psittacosis/ Ornithosis             Spleen and Lung                                                      On ice

Ranikhet disease                    a. Spleen                                                                    On ice
b. Lung, Spleen, Trachea, Brain                        10% formaline
c. Ailing birds
d. Serum samples ( 10 Birds)

Marek’s disease                     a. Plucked feathers with roots                            Dry / On ice
b. Liver, spleen, tumours,
Sciatic and vagus nerves                              10% formaline
c. Serum (10)

ILT / IB                                 a. Trachea and Lungs                                                  On ice
b. Trachea and Lungs                                                    10% formaline
c. paired sera

Fowl pox                                Pox lesions                                                     50% GPB
Lymphoid leucosis                  a. Liver, spleen, tumors                                               10% formaline
b. Long bones in osteoporosis form

Avian encephalitis                   a. Brain                                                              On ice/ 50% GPB

IBD                                        a. Bursa of fabricius, spleen                             On ice/50% GPB

DIAGNOSTIC MATERIALS  FOR PARASITIC DISEASES

Endoparasites                        Faecal sample                                      No preservative /
Parasites or its ploglottids                   5 % formalin

Coccidiosis                             Faecal sample                                      2.5% Pot.dichromate                                                  Intestinal scrapings

Mange                                                Deep skin scrapings                            in a dry paper

Ticks                                       Parasites as such                                  in a dry bottle

DIAGNOSTIC MATERIALS  FOR BLOOD PROTOZOA

Babesiosis                               Thin Blood smears                              Air dried or alcohol Anaplasmosis                                                                                        fixed
Theilariasis

Trypanosomiasis                   Thick blood smears                             Air dried

MATERIALS  TO BE SENT FOR TOXICOSES OR POISONING

Toxicosis                                 Feed sample                                        No preservative
                                                Pieces of liver, spleen                          10 % formalin

Poisoning                                Stomach or intestinal contents            On ice
Urine sample                                       On ice
Suspected food material                     On ice
Pieces of liver, spleen, kidney             In rectified spirit or
Saturated salt solution
Particulars of  Vaccines  produced at V.B.R.I.

Sl.
No.

Name of the vaccine

Vaccine production
range per year in lakh doses

Packing (Doses)

Description of vaccine

Storage temperature

Dose

Route of inoculation

1.

HS Vaccine

80 – 90

50

Inactivated , alum precipitated

40C

5 ml 

S/C

2.

BQ Vaccine

30 – 35

50

Inactivated , alum precipitated

40C

5 ml

S/C

3.

ET Vaccine

90 – 100

100

Alum precipitated epsilon toxoid

40C

2.5 ml

S/C

4.

Anthrax Spore Vaccine

5 – 8

100

 Live spore vaccine in 50%   glycerine saline

40C

1 ml

S/C

5

Sheep Pox TC Vaccine

70 – 80

100

Attenuated  freeze dried

- 200C

0.1 ml

Intra dermal

6

PPR TC Vaccine

90 – 100

100

Attenuated  freeze dried

- 200C

1 ml

S/C

7

RD ‘K’ Vaccine

160- 180

200

Live freeze dried

- 200C

0.5 ml

S/C

8

RD ‘FI’ Vaccine

15 – 20

100

Live freeze dried

- 200C

1 or 2 drops

Intra ocular  / Intranasal

9

Fowl Pox Vaccine

80 - 100

200

Live freeze dried

- 200C

2 pricks

Wing web

10

Anti Rabies TC Vaccine

-

Single /
Multiple

Inactivated , adsorbed on aluminum hydroxide gel

   2 - 80C

1 ml

S/C or I/M

            Note: All the freeze dried viral vaccines are to be reconstituted before vaccination with chilled normal saline / PBS

HAEMORRHAGIC SEPTICAEMIA VACCINE

Formalin inactivated, alum precipitated vaccine containing Pasteurella multocida P52 strain.

Guide lines for usage: 

Shake the bottle well before use

Sterile needles & syringes should be used for every withdrawal and injection
Schedule of vaccination:
Prophylactic vaccination is recommended in all the cattle and buffaloes above 4 months of age before onset of the monsoon and vaccination may be repeated in endemic areas.
Dosage: Cattle, Buffaloes -   5 ml              

Route of administration:

Subcutaneous route preferably at mid neck region.

Storage:  

Vaccine should be stored at 4º C. The shelf life is 6 months from the date of manufacture when stored at proper temperature. The vaccine should not be allowed to freeze

Immunity:

Immunity persists for nearly 4 - 6 months. 

Packing
           

50 doses / 250 ml in polypropylene bottles

BLACK QUARTER VACCINE

Formalin inactivated, alum precipitated vaccine containing Clostridium chauvoei bacterial mass

Guide lines for usage :
Shake the bottle well before use
Sterile needles & syringes should be used for every withdrawal and injection

Schedule of vaccination :
Prophylactic vaccination is recommended in cattle and buffaloes above 4 months of age one month before the onset of the disease in endemic areas

Storage:
Vaccine should be stored at 4º C. The shelf life is 6 months from the date of manufacture when stored at proper temperature. The vaccine should not be allowed to freeze

Dosage :  
Cattle and buffaloes - 5ml

Administration :
Subcutaneous route preferably at  mid neck region.
Immunity :
Immunity persists for around 9 months.

Packing :

50 doses / 250 ml in polypropylene bottles

ENTERO TOXAEMIA VACCINE

Formalin inactivated, alum precipitated vaccine containing Clostridium perfringens type D bacterial mass along with Epsilon toxoid  .

Dose  :  
Sheep & Goats  - 2.5ml
Route of Administration:
Subcutaneous route at the flank region

Guide lines for administration:

  • The animals should be free from worm burden prior to vaccination to establish sufficient   

    immunity

  • Shake the bottle well before and in between vaccinations to ensure uniform mixing of the   contents
  • Sterile needles & syringes should be used for every withdrawal and injection
  • Hypersensitivity may occur in rare cases where  immediate treatment with antihistamines is 

    advocated
Schedule of vaccination  :

    •   Prophylactic vaccinations is advised in all sheep and goats above 3 months of age, and one   month in  advance before the outbreak season
    •   Booster vaccination is recommended   for the lambs after 14 days
    •   Yearly vaccinations are recommended in adult animals.

Immunity :
Immunity persists for 9 months.
Storage :
Vaccine should be stored at  4 0- 8 0C. The vaccine shelf life is 9 months from the date of manufacture if stored at proper temperature. The vaccine should not be allowed to freeze

Packing :
         

100 doses /250 ml Poly propylene bottle

ANTHRAX SPORE VACCINE

Anthrax vaccine is a suspension of uncapsulated avirulent strain of Bacillus anthracis sporesin
50 % glycerine saline.

Guide lines for usage

  • Precautions have to be taken to guard against infection to self while vaccinating the animals as  the vaccine contains live spores
  • Shake the bottle well before use
  • Physical exertion should be avoided following vaccination for 3 days.
  • Do not vaccinate the animals 60 days before slaughter
  • Sterile needles & syringes should be used for every withdrawal and injection

Schedule of vaccination:

  • Vaccination is advised in  endemic areas
  • Vaccinate the animals preferably in monsoon season or prior before the season the disease  usually occurs
  • Affected animals should not be vaccinated
    Dosage :

Cattle, Buffaloes, Sheep and Goats  – 1.0 ml

Route of administration:
Subcutaneous

Storage:
Vaccine should be stored at 4º C. The shelf life is 1 year from the date of manufacture when stored at proper temperature. The vaccine should not be allowed to freeze

Immunity:
Immunity period is one year.

Packing

100 doses / 250 ml in polypropylene bottles 

SHEEP POX TISSUE CULTURE VACCINE

Live attenuated freeze-dried vaccine prepared by growing   “Romanian fenner” strain of Sheep pox virus on secondary lamb testicular cells.

Guide lines for Usage:

  • The contents of each vial are reconstituted in 10 ml of sterile, chilled normal saline. The reconstituted vaccine should be used within 2 hours.
  • The vaccine evokes thermal and / or local cutaneous reaction following vaccination without generalization. Takes appear at the site of inoculation after 4 days of vaccination
  • In some cases local reaction may progress to ulceration.
  • Sterile needles & syringes should be used for every withdrawal and injection

  Schedule of vaccination

  • Prophylactic vaccination in sheep is recommended one month before the onset of the disease
  • Vaccinate the animals above 3 months of age

Dosage:

Sheep -   0.1 ml

Route of administration:

Intra dermal -  the preferred site is on the tip of the ear .

Storage:
The vaccine should be stored at -20 ºC. The keeping quality of the vaccine is 1 year at -20º C.

Immunity:

More than 1 year.  

Packing:

 

5 ml glass vials containing 100 doses of freeze dried Sheep pox vaccine

PPR TISSUE CULTURE VACCINE

Live attenuated freeze-dried vaccine prepared by growing Sungri strain of PPR virus on Vero cell lines.

Guidelines of Usage:

  • The contents of each vial should be reconstituted in 100 ml of sterile, chilled normal saline/PBS.
  •  The reconstituted vaccine should be kept on ice protected from direct sun light and should be used within 2 hours.
  • Sterile needles & syringes should be used for every withdrawal and injection

Schedule of vaccination:

  • Prophylactic Vaccination in sheep and goats is recommended one month before the onset of outbreak season
  • Vaccinate all the sheep and goats above 3 months of age.

Dosage:
Sheep and goats – 1ml of reconstituted vaccine

Route of administration:
Subcutaneous

Storage: 
The vaccine should be stored at -20°C temperature. The shelf life of the freeze dried vaccine is more than one year if properly stored at –20°C.

Immunity :
The immunity is around  3 years

Packing:
                               

5 ml glass vials containing 100 doses of freeze dried PPR vaccine      

RANIKHET DISEASE “K” VACCINE 

Live freeze dried vaccine prepared by growing Komorov strain of RD virus in Egg embryos .

 Guide lines for usage:

  • The contents of each vial are to be reconstituted in 100 ml of sterile, chilled normal saline.
  • The reconstituted vaccine should be kept on ice, protected from the direct sunlight and should be used within 2  hours
  • Vaccination should be conducted only in healthy flocks   
  • Sterile needles & syringes should be used for every withdrawal and injection

Storage:
The vaccine should be stored at   -20 °C temperature. The freeze dried vaccine shelf life is one year if stored at  -20 °C

Schedule of vaccination
6-8 weeks old birds are to be vaccinated

Immunity:
Life long

Dosage
0.5 ml of reconstituted vaccine

Route of administration:
Subcutaneous route in adult birds

Packing:
     
2 ml glass vial containing 200 doses of freeze dried vaccine.

RANIKHET DISEASE “F” VACCINE

Live freeze dried vaccine prepared by growing F strain of RD virus in Egg embryos .

Guide lines for usage:

  • The contents of each vial is reconstituted in 10 ml of sterile, chilled normal saline.
  • The reconstituted vaccine should be protected from direct sun light and used within 2 hours.

Schedule of vaccination:
One week old chicks are to be vaccinated.

Dosage:
One or two drops of reconstituted vaccine.

Route of administration:
Intra nasal /  Intra ocular

Storage:
The vaccine should be stored at -20 °C.  Shelf life of the vaccine is one year at -20 °C

Packing:

2 ml glass vial containing 100 doses of freeze dried vaccine.

FOWL POX VACCINE

Live freeze dried vaccine prepared by growing Bordette strain of of Fowl pox virus in Egg embryos .

Guide lines for usage:

  • Each vial is reconstituted with 3 ml of 50% sterile chilled glycerin saline.  The reconstituted vaccine is kept on ice and should be used within 2 hrs. 
  • Healthy birds above 6 weeks age group are to be vaccinated and the vaccinated birds are examined for ‘takes’ seven days after vaccination. 

 

  • Sterile needles & syringes should be used for every withdrawal and injection

Schedule of vaccination:
This vaccine is used in poultry of above six weeks age.

Storage:
The vaccine should be stored at -20 0C.  The shelf life of vaccine is about 1 year at -20 0C

Dosage & Administration:      
The birds are vaccinated by prick method at wing web region by applying two pricks at one cm apart.

Immunity:
This vaccine gives immunity for about six months.

Packing:
2 ml glass vial containing 200 doses of freeze dried vaccine.

ANTI RABIES TISSUE CULTURE VACCINE

BHK 21 cell based Beta propiolactone inactivated Aluminium hydroxide gel adsorbed vaccine containing PV3462 strain of Rabies virus. This vaccine can be used for pre and post exposure therapy in all rabies susceptible animals.

Guide lines for usage.

  • Weak and debilitated animals should not be vaccinated.
  • Female animals should not be vaccinated when they are in heat or in advanced pregnancy.                                                                 
  • Corticosteroids are contraindicated during Post-bite treatment, as they interfere the immune  response system to rabies viral antigens.
  • Shake the bottle well before use
  • Sterile needles & syringes should be used for every withdrawal and injection

Schedule of Vaccination:  
Prophylactic vaccination is advised every year starting from 4 months age.   
Post-exposure therapy is as follows      
1st   Dose:  0    day (Immediately after dog bite)
2nd  Dose:  3rd  day
3rd  Dose:  7th  day
4th  Dose: 14th  day
5th   Dose: 28th  day
6th  Dose: 90th  day (Booster)

Dosage:
Dogs, cattle, sheep, goats and all other susceptible animals - 1.0 ml

Route of administration:
Sub cutaneous or Intra muscular.

Storage
The vaccine should be stored at 2-8°C temperature. The shelf life of the vaccine is one year at proper storage. The vaccine should not be allowed to freeze.

Immunity:
Duration of immunity is supposed to be 2 years. However annual vaccination is recommended

Packing:
Single dose - glass vial of 1.0 ml
Multi dose -   glass vial of 5.0 ml